Functionalized Poplar Powder as a Support Material for Immobilization of Enoate Reductase and a Cofactor Regeneration System.
Identifieur interne : 000953 ( Main/Exploration ); précédent : 000952; suivant : 000954Functionalized Poplar Powder as a Support Material for Immobilization of Enoate Reductase and a Cofactor Regeneration System.
Auteurs : Han Li [République populaire de Chine] ; Xiumei Cui [République populaire de Chine] ; Liangyu Zheng [République populaire de Chine]Source :
- Journal of microbiology and biotechnology [ 1738-8872 ] ; 2019.
Descripteurs français
- KwdFr :
- Coenzymes (métabolisme), Enzymes immobilisées (métabolisme), Glucose 6-phosphate dehydrogenase (métabolisme), Glutaraldéhyde (MeSH), Immobilisation (méthodes), Lignine (composition chimique), Oxidoreductases (métabolisme), Populus (composition chimique), Régénération (MeSH), Stabilité enzymatique (MeSH), Éthylènediamines (MeSH).
- MESH :
- composition chimique : Lignine, Populus.
- métabolisme : Coenzymes, Enzymes immobilisées, Glucose 6-phosphate dehydrogenase, Oxidoreductases.
- méthodes : Immobilisation.
- Glutaraldéhyde, Régénération, Stabilité enzymatique, Éthylènediamines.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Lignin.
- chemical , metabolism : Coenzymes, Enzymes, Immobilized, Glucosephosphate Dehydrogenase, Oxidoreductases.
- chemistry : Populus.
- methods : Immobilization.
- Enzyme Stability, Ethylenediamines, Glutaral, Regeneration.
Abstract
In this study, functionalized poplar powder (FPP) was used as a support material for the immobilization of enoate reductase (ER) and glucose-6-phosphate dehydrogenase (GDH) by covalent binding. Under optimal conditions, the immobilization efficiency of ER-FPP and GDH-FPP was 95.1% and 84.7%, and the activity recovery of ER and GDH was 47.5% and 37.8%, respectively. Scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) analysis indicated that FPP was a suitable carrier for enzyme immobilization. ER-FPP and GDH-FPP exhibit excellent thermal stabilities and superior reusability. Especially, ER-FPP and GDH-FPP enable the continuous conversion of 4-(4-Methoxyphenyl)-3-buten-2-one with NAD+ recycling. While the immobilization strategies established here were simple and inexpensive, they exploited a new method for the immobilization and application of ER and its cofactor recycling system.
DOI: 10.4014/jmb.1811.11054
PubMed: 30954031
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Functionalized Poplar Powder as a Support Material for Immobilization of Enoate Reductase and a Cofactor Regeneration System.</title><author><name sortKey="Li, Han" sort="Li, Han" uniqKey="Li H" first="Han" last="Li">Han Li</name><affiliation wicri:level="4"><nlm:affiliation>Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, College of Life Sciences, Jilin University, Changchun 130012, P. R. China.</nlm:affiliation><orgName type="university">Université de Jilin</orgName><country>République populaire de Chine</country><placeName><settlement type="city">Changchun</settlement><region type="province">Jilin</region></placeName></affiliation></author><author><name sortKey="Cui, Xiumei" sort="Cui, Xiumei" uniqKey="Cui X" first="Xiumei" last="Cui">Xiumei Cui</name><affiliation wicri:level="4"><nlm:affiliation>Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, College of Life Sciences, Jilin University, Changchun 130012, P. R. China.</nlm:affiliation><orgName type="university">Université de Jilin</orgName><country>République populaire de Chine</country><placeName><settlement type="city">Changchun</settlement><region type="province">Jilin</region></placeName></affiliation></author><author><name sortKey="Zheng, Liangyu" sort="Zheng, Liangyu" uniqKey="Zheng L" first="Liangyu" last="Zheng">Liangyu Zheng</name><affiliation wicri:level="4"><nlm:affiliation>Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, College of Life Sciences, Jilin University, Changchun 130012, P. R. China.</nlm:affiliation><orgName type="university">Université de Jilin</orgName><country>République populaire de Chine</country><placeName><settlement type="city">Changchun</settlement><region type="province">Jilin</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:30954031</idno><idno type="pmid">30954031</idno><idno type="doi">10.4014/jmb.1811.11054</idno><idno type="wicri:Area/Main/Corpus">000957</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000957</idno><idno type="wicri:Area/Main/Curation">000957</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000957</idno><idno type="wicri:Area/Main/Exploration">000957</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Functionalized Poplar Powder as a Support Material for Immobilization of Enoate Reductase and a Cofactor Regeneration System.</title><author><name sortKey="Li, Han" sort="Li, Han" uniqKey="Li H" first="Han" last="Li">Han Li</name><affiliation wicri:level="4"><nlm:affiliation>Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, College of Life Sciences, Jilin University, Changchun 130012, P. R. China.</nlm:affiliation><orgName type="university">Université de Jilin</orgName><country>République populaire de Chine</country><placeName><settlement type="city">Changchun</settlement><region type="province">Jilin</region></placeName></affiliation></author><author><name sortKey="Cui, Xiumei" sort="Cui, Xiumei" uniqKey="Cui X" first="Xiumei" last="Cui">Xiumei Cui</name><affiliation wicri:level="4"><nlm:affiliation>Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, College of Life Sciences, Jilin University, Changchun 130012, P. R. China.</nlm:affiliation><orgName type="university">Université de Jilin</orgName><country>République populaire de Chine</country><placeName><settlement type="city">Changchun</settlement><region type="province">Jilin</region></placeName></affiliation></author><author><name sortKey="Zheng, Liangyu" sort="Zheng, Liangyu" uniqKey="Zheng L" first="Liangyu" last="Zheng">Liangyu Zheng</name><affiliation wicri:level="4"><nlm:affiliation>Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, College of Life Sciences, Jilin University, Changchun 130012, P. R. China.</nlm:affiliation><orgName type="university">Université de Jilin</orgName><country>République populaire de Chine</country><placeName><settlement type="city">Changchun</settlement><region type="province">Jilin</region></placeName></affiliation></author></analytic><series><title level="j">Journal of microbiology and biotechnology</title><idno type="eISSN">1738-8872</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coenzymes (metabolism)</term><term>Enzyme Stability (MeSH)</term><term>Enzymes, Immobilized (metabolism)</term><term>Ethylenediamines (MeSH)</term><term>Glucosephosphate Dehydrogenase (metabolism)</term><term>Glutaral (MeSH)</term><term>Immobilization (methods)</term><term>Lignin (chemistry)</term><term>Oxidoreductases (metabolism)</term><term>Populus (chemistry)</term><term>Regeneration (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Coenzymes (métabolisme)</term><term>Enzymes immobilisées (métabolisme)</term><term>Glucose 6-phosphate dehydrogenase (métabolisme)</term><term>Glutaraldéhyde (MeSH)</term><term>Immobilisation (méthodes)</term><term>Lignine (composition chimique)</term><term>Oxidoreductases (métabolisme)</term><term>Populus (composition chimique)</term><term>Régénération (MeSH)</term><term>Stabilité enzymatique (MeSH)</term><term>Éthylènediamines (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Lignin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Coenzymes</term><term>Enzymes, Immobilized</term><term>Glucosephosphate Dehydrogenase</term><term>Oxidoreductases</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Lignine</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Immobilization</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Coenzymes</term><term>Enzymes immobilisées</term><term>Glucose 6-phosphate dehydrogenase</term><term>Oxidoreductases</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Immobilisation</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Enzyme Stability</term><term>Ethylenediamines</term><term>Glutaral</term><term>Regeneration</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Glutaraldéhyde</term><term>Régénération</term><term>Stabilité enzymatique</term><term>Éthylènediamines</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In this study, functionalized poplar powder (FPP) was used as a support material for the immobilization of enoate reductase (ER) and glucose-6-phosphate dehydrogenase (GDH) by covalent binding. Under optimal conditions, the immobilization efficiency of ER-FPP and GDH-FPP was 95.1% and 84.7%, and the activity recovery of ER and GDH was 47.5% and 37.8%, respectively. Scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) analysis indicated that FPP was a suitable carrier for enzyme immobilization. ER-FPP and GDH-FPP exhibit excellent thermal stabilities and superior reusability. Especially, ER-FPP and GDH-FPP enable the continuous conversion of 4-(4-Methoxyphenyl)-3-buten-2-one with NAD<sup>+</sup> recycling. While the immobilization strategies established here were simple and inexpensive, they exploited a new method for the immobilization and application of ER and its cofactor recycling system.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30954031</PMID><DateCompleted><Year>2019</Year><Month>06</Month><Day>17</Day></DateCompleted><DateRevised><Year>2019</Year><Month>06</Month><Day>17</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1738-8872</ISSN><JournalIssue CitedMedium="Internet"><Volume>29</Volume><Issue>4</Issue><PubDate><Year>2019</Year><Month>Apr</Month><Day>28</Day></PubDate></JournalIssue><Title>Journal of microbiology and biotechnology</Title><ISOAbbreviation>J Microbiol Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Functionalized Poplar Powder as a Support Material for Immobilization of Enoate Reductase and a Cofactor Regeneration System.</ArticleTitle><Pagination><MedlinePgn>607-616</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.4014/jmb.1811.11054</ELocationID><Abstract><AbstractText>In this study, functionalized poplar powder (FPP) was used as a support material for the immobilization of enoate reductase (ER) and glucose-6-phosphate dehydrogenase (GDH) by covalent binding. Under optimal conditions, the immobilization efficiency of ER-FPP and GDH-FPP was 95.1% and 84.7%, and the activity recovery of ER and GDH was 47.5% and 37.8%, respectively. Scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) analysis indicated that FPP was a suitable carrier for enzyme immobilization. ER-FPP and GDH-FPP exhibit excellent thermal stabilities and superior reusability. Especially, ER-FPP and GDH-FPP enable the continuous conversion of 4-(4-Methoxyphenyl)-3-buten-2-one with NAD<sup>+</sup> recycling. While the immobilization strategies established here were simple and inexpensive, they exploited a new method for the immobilization and application of ER and its cofactor recycling system.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Han</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, College of Life Sciences, Jilin University, Changchun 130012, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cui</LastName><ForeName>Xiumei</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, College of Life Sciences, Jilin University, Changchun 130012, P. R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Liangyu</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, College of Life Sciences, Jilin University, Changchun 130012, P. R. China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Korea (South)</Country><MedlineTA>J Microbiol Biotechnol</MedlineTA><NlmUniqueID>9431852</NlmUniqueID><ISSNLinking>1017-7825</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003067">Coenzymes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004800">Enzymes, Immobilized</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005029">Ethylenediamines</NameOfSubstance></Chemical><Chemical><RegistryNumber>11132-73-3</RegistryNumber><NameOfSubstance UI="C036909">lignocellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>60V9STC53F</RegistryNumber><NameOfSubstance UI="C031234">ethylenediamine</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.-</RegistryNumber><NameOfSubstance UI="D010088">Oxidoreductases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.1.1.49</RegistryNumber><NameOfSubstance UI="D005954">Glucosephosphate Dehydrogenase</NameOfSubstance></Chemical><Chemical><RegistryNumber>T3C89M417N</RegistryNumber><NameOfSubstance UI="D005976">Glutaral</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D003067" MajorTopicYN="N">Coenzymes</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004795" MajorTopicYN="N">Enzyme Stability</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004800" MajorTopicYN="N">Enzymes, Immobilized</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005029" MajorTopicYN="N">Ethylenediamines</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005954" MajorTopicYN="N">Glucosephosphate Dehydrogenase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005976" MajorTopicYN="N">Glutaral</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007103" MajorTopicYN="N">Immobilization</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010088" MajorTopicYN="N">Oxidoreductases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012038" MajorTopicYN="N">Regeneration</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Enoate reductase</Keyword><Keyword MajorTopicYN="N">cofactor regeneration system</Keyword><Keyword MajorTopicYN="N">covalent immobilization</Keyword><Keyword MajorTopicYN="N">functionalized poplar powder</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>4</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>6</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>4</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30954031</ArticleId><ArticleId IdType="pii">10.4014/jmb.1811.11054</ArticleId><ArticleId IdType="doi">10.4014/jmb.1811.11054</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Jilin</li></region><settlement><li>Changchun</li></settlement><orgName><li>Université de Jilin</li></orgName></list><tree><country name="République populaire de Chine"><region name="Jilin"><name sortKey="Li, Han" sort="Li, Han" uniqKey="Li H" first="Han" last="Li">Han Li</name></region><name sortKey="Cui, Xiumei" sort="Cui, Xiumei" uniqKey="Cui X" first="Xiumei" last="Cui">Xiumei Cui</name><name sortKey="Zheng, Liangyu" sort="Zheng, Liangyu" uniqKey="Zheng L" first="Liangyu" last="Zheng">Liangyu Zheng</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000953 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000953 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:30954031
|texte= Functionalized Poplar Powder as a Support Material for Immobilization of Enoate Reductase and a Cofactor Regeneration System.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:30954031" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |